Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50

doi:10.3390/microorganisms11040854

	Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50
	Yuan, Cansheng 1; Zhao, Lingling 2; Tong, Lu 3; Wang, Lin 1; Ke, Zhuang 1; Yang, Ying 1; He, Jian 1,2
	2023-04
发表期刊	MICROORGANISMS
卷号	11 期号:4
摘要	Picolinic acid (PA) is a typical mono-carboxylated pyridine derivative produced by human/animals or microorganisms which could be served as nutrients for bacteria. Most Bordetella strains are pathogens causing pertussis or respiratory disease in humans and/or various animals. Previous studies indicated that Bordetella strains harbor the PA degradation pic gene cluster. However, the degradation of PA by Bordetella strains remains unknown. In this study, a reference strain of genus Bordetella, B. bronchiseptica RB50, was investigated. The organization of pic gene cluster of strain RB50 was found to be similar with that of Alcaligenes faecalis, in which the sequence similarities of each Pic proteins are between 60% to 80% except for PicB2 (47% similarity). The 3,6-dihydroxypicolinic acid (3,6DHPA) decarboxylase gene (BB0271, designated as picC(RB50)) of strain RB50 was synthesized and over-expressed in E. coli BL21(DE3). The PicC(RB50) showed 75% amino acid similarities against known PicC from Alcaligenes faecalis. The purified PicC(RB50) can efficiently transform 3,6DHPA to 2,5-dihydroxypyridine. The PicC(RB50) exhibits optimal activities at pH 7.0, 35 degrees C, and the K-m and k(cat) values of PicC(RB50) for 3,6DHPA were 20.41 +/- 2.60 mu M and 7.61 +/- 0.53 S-1, respectively. The present study provided new insights into the biodegradation of PA by pathogens of Bordetella spp.
关键词	picolinic acid PICOLINIC-ACID decarboxylase 2-PICOLINIC ACID Bordetella bronchiseptica RB50 DEGRADATION biodegradation NITROBENZENE pathogen
DOI	10.3390/microorganisms11040854
收录类别	SCIE
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000978412000001
出版者	MDPI
原始文献类型	Article
EISSN	2076-2607
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.library.ouchn.edu.cn/handle/39V7QQFX/168637
专题	国家开放大学江苏分部
通讯作者	He, Jian
作者单位	1.Jiangsu Open Univ, Coll Rural Revitalizat, Nanjing 210036, Peoples R China; 2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China; 3.Suzhou Kaisiling Environm Scitechnol Co Ltd, Suzhou 215413, Peoples R China
第一作者单位	国家开放大学江苏分部
通讯作者单位	国家开放大学江苏分部
第一作者的第一单位	国家开放大学江苏分部
推荐引用方式 GB/T 7714	Yuan, Cansheng,Zhao, Lingling,Tong, Lu,et al. Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50[J]. MICROORGANISMS,2023,11(4).
APA	Yuan, Cansheng.,Zhao, Lingling.,Tong, Lu.,Wang, Lin.,Ke, Zhuang.,...&He, Jian.(2023).Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50.MICROORGANISMS,11(4).
MLA	Yuan, Cansheng,et al."Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50".MICROORGANISMS 11.4(2023).