Overexpression of TaLEA Gene from Tamarix androssowii Improves Salt and Drought Tolerance in Transgenic Poplar (Populus simonii x P. nigra)

doi:10.1371/journal.pone.0067462

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	Overexpression of TaLEA Gene from Tamarix androssowii Improves Salt and Drought Tolerance in Transgenic Poplar (Populus simonii x P. nigra)
	Gao, Weidong 1; Bai, Shuang 2,5; Li, Qingmei 1; Gao, Caiqiu 2; Liu, Guifeng 2; Li, Guangde 3; Tan, Feili 4
	2013-06-28
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:6
摘要	Late embryogenesis abundant (LEA) genes were confirmed to confer resistance to drought and water deficiency. An LEA gene from Tamarix androssowii (named TaLEA) was transformed into Xiaohei poplar (Populus simonii x P. nigra) via Agrobacterium. Twenty-five independent transgenic lines were obtained that were resistant to kanamycin, and 11 transgenic lines were randomly selected for further analysis. The polymerase chain reaction (PCR) and ribonucleic acid (RNA) gel blot indicated that the TaLEA gene had been integrated into the poplar genome. The height growth rate, malondialdehyde (MDA) content, relative electrolyte leakage and damages due to salt or drought to transgenic and non-transgenic plants were compared under salt and drought stress conditions. The results showed that the constitutive expression of the TaLEA gene in transgenic poplars could induce an increase in height growth rate and a decrease in number and severity of wilted leaves under the salt and drought stresses. The MDA content and relative electrolyte leakage in transgenic lines under salt and drought stresses were significantly lower compared to those in non-transgenic plants, indicating that the TaLEA gene may enhance salt and drought tolerance by protecting cell membranes from damage. Moreover, amongst the lines analyzed for stress tolerance, the transgenic line 11 (T11) showed the highest tolerance levels under both salinity and drought stress conditions. These results indicated that the TaLEA gene could be a salt and drought tolerance candidate gene and could confer a broad spectrum of tolerance under abiotic stresses in poplars.
DOI	10.1371/journal.pone.0067462
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000321148400084
出版者	PUBLIC LIBRARY SCIENCE
原始文献类型	Article
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.library.ouchn.edu.cn/handle/39V7QQFX/25035
专题	国家开放大学
通讯作者	Li, Qingmei
作者单位	1.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China; 2.Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China; 3.Open Univ China, Sch Agroforestry & Med, Beijing, Peoples R China; 4.Zhanjiang Normal Univ, Sch Life Sci & Technol, Zhanjiang, Peoples R China; 5.Beijing Ba Da Chu Pk, Engn & Garden Dept, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, Weidong,Bai, Shuang,Li, Qingmei,et al. Overexpression of TaLEA Gene from Tamarix androssowii Improves Salt and Drought Tolerance in Transgenic Poplar (Populus simonii x P. nigra)[J]. PLOS ONE,2013,8(6).
APA	Gao, Weidong.,Bai, Shuang.,Li, Qingmei.,Gao, Caiqiu.,Liu, Guifeng.,...&Tan, Feili.(2013).Overexpression of TaLEA Gene from Tamarix androssowii Improves Salt and Drought Tolerance in Transgenic Poplar (Populus simonii x P. nigra).PLOS ONE,8(6).
MLA	Gao, Weidong,et al."Overexpression of TaLEA Gene from Tamarix androssowii Improves Salt and Drought Tolerance in Transgenic Poplar (Populus simonii x P. nigra)".PLOS ONE 8.6(2013).