Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase

doi:10.1007/s00216-024-05630-0

	Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase
	Yu, Shuaibing 1,2; Li, Lianzhi 3; Kong, Qiyun 4; Zhang, Wenqi 1; Chen, Huan 2; Zhang, Xueji 5; Kong, Jinming 2
	2024-11-08
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
摘要	The instability of the signal intensity of fluorescent biosensors and the false signals have been significant factors affecting the performance of biosensors. Herein, a novel signaling system is devised through the application of reversible addition-fragmentation chain transfer (RAFT) polymerization with monomers containing the tetraphenylethylene (TPE) groups. TPE exhibits an aggregation-induced emission (AIE) phenomenon in certain solvents, mainly due to the blockage of the rotation of its four benzene rings, which also exist in the aggregated state. With this property, a series of molecules are modified based on click chemistry for RAFT polymerization using Fe3O4 magnetic beads as the carriers, and stable aggregated luminescent TPE polymers are formed on the surface of magnetic beads to realize the transformation of fluorescence signal from 0 to 1. In addition, the fluorescence signal demonstrates a positive correlation with alkaline phosphatase (ALP) activity, which can be quantified by measuring the fluorescence intensity. The biosensor exhibits high sensitivity and good linearity in the range of 0.1-5 U/L, with a LOD of 0.079 U/L. Furthermore, the designed strategy demonstrated satisfactory performance in the quantitative determination of ALP activity in serum samples, indicating that the signaling system developed by combining RAFT polymerization and AIE molecules has an important application in the field of fluorescent biosensors.
关键词	Aggregation-induced emission RAFT polymerization Controlled chain growth luminescence ALP Fluorescent biosensor
其他关键词	INDUCED EMISSION ; NANOPARTICLES ; LIQUID ; SENSOR ; CLICK
DOI	10.1007/s00216-024-05630-0
收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:001349851600001
出版者	SPRINGER HEIDELBERG
原始文献类型	Article ; Early Access
EISSN	1618-2650
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.library.ouchn.edu.cn/handle/39V7QQFX/172553
专题	国家开放大学江苏分部
通讯作者	Kong, Jinming
作者单位	1.Jiangsu Open Univ, Coll Environm & Ecol, Nanjing 210017, Peoples R China; 2.Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Peoples R China; 3.Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Peoples R China; 4.Hwa Chong Inst, 661 Bukit Timah Rd, Singapore 269734, Singapore; 5.Shenzhen Univ, Hlth Sci Ctr, Sch Biomed Engn, Shenzhen 518060, Peoples R China
第一作者单位	国家开放大学江苏分部
第一作者的第一单位	国家开放大学江苏分部
推荐引用方式 GB/T 7714	Yu, Shuaibing,Li, Lianzhi,Kong, Qiyun,et al. Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2024.
APA	Yu, Shuaibing.,Li, Lianzhi.,Kong, Qiyun.,Zhang, Wenqi.,Chen, Huan.,...&Kong, Jinming.(2024).Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Yu, Shuaibing,et al."Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2024).